rabbit anti nectin2  (Proteintech)

Journal: Scientific reports

Article Title: Single-cell RNA sequencing unveils tumor heterogeneity and immune microenvironment between subungual and plantar melanoma.

doi: 10.1038/s41598-024-57640-8

Figure Lengend Snippet: Figure 5. T/NK cells interact with melanocytes via the TIGIT-NECTIN2 axis. (A) Bar plot showing the number and strength of interactions between melanocytes and T/NK cells in PM1 and SM1. (B) Differences in interactions in the information flow of PM1 and SM1. (C) Dot plot showing receptor-ligand pair analysis of the interactions between melanocytes and T/NK cells. (D,E) Cell chat showing the interactions between TIGIT and its ligand in PM1 and SM1. The thickness of the lines represents strength. (F) Violin plots showing the expression intensity of TIGIT and its ligand NECTIN2/PVR in melanocytes subpopulations and T/NK cells subpopulations. (G,H) Pseudotime trajectory demonstrating the transcriptome lineage of 8T/NK cells subpopulations. Colors indicate pseudotime progression. (I) Line plot showing the development trend of module 4 in pseudotime trajectory. (J) The development trend of 4 modules of T/NK cells in pseudotime trajectory. (K) Immunofluorescence staining of TIGIT in PM and SM.

Article Snippet: After 30 min blocking in 3% bovine serum albumin (BSA), tissues were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-EFNA1 antibody (1:200, Cusabio Cat# CSB-PA002367), rabbit anti-CD226 antibody (1:200, Cusabio Cat# CSB-PA006679), rabbit anti-TIGIT antibody (1:100, Cusabio Cat# CSB-PA675446LA01HU), and rabbit anti-NECTIN2 antibody (1:200, Proteintech Cat No. 27171–1-AP).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Scientific reports

Article Title: Single-cell RNA sequencing unveils tumor heterogeneity and immune microenvironment between subungual and plantar melanoma.

doi: 10.1038/s41598-024-57640-8

Figure Lengend Snippet: Figure 6. The expression of CD226-NECTIN2 axis in melanocytes and T/NK cells. (A,B) Cell chat showing the interactions between CD226 and its ligand in PM1 and SM1. The thickness of the lines represents strength. (C) Violin plots showing the expression intensity of CD226 and its ligand NECTIN2/PVR in melanocytes subpopulations and T/NK cells subpopulations. (D) Hematoxylin–eosin (HE) staining of PM and SM (× 100). Immunofluorescence staining of CD226 and NECTIN2 in PM and SM. Triple immunofluorescence staining of TIGIT, CD226, and NECTIN2. (E) SCENIC showing the main TFs of CD8+ Cytotoxic. (F) The interaction of TIGIT family receptors and ligands.

Article Snippet: After 30 min blocking in 3% bovine serum albumin (BSA), tissues were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-EFNA1 antibody (1:200, Cusabio Cat# CSB-PA002367), rabbit anti-CD226 antibody (1:200, Cusabio Cat# CSB-PA006679), rabbit anti-TIGIT antibody (1:100, Cusabio Cat# CSB-PA675446LA01HU), and rabbit anti-NECTIN2 antibody (1:200, Proteintech Cat No. 27171–1-AP).

Techniques: Expressing, Staining, Immunofluorescence

Journal: Journal for Immunotherapy of Cancer

Article Title: Interactions between LAMP3+ dendritic cells and T-cell subpopulations promote immune evasion in papillary thyroid carcinoma

doi: 10.1136/jitc-2024-008983

Figure Lengend Snippet: The recruitment of LAMP3 + DCs by tumor cells promotes PTC clinical progression. Heatmaps (A) and circle (B) plots show the comparison of interaction quantity and interaction strength between thyrocytes and cDCs between non-progressive PTC and progressive PTC. Red indicated that the increase of communication in the latter. (C) The interaction between thyrocytes and cDCs is more numerous and stronger in Group B. (D) Summary of selected ligand-receptor interactions between thyrocytes and cDC-C3 cells in the two groups. (E) Circle plots showing the interaction between NECTIN3-NECTIN2 ligand-receptor pairs in the cDCs and thyrocytes. (F) The expression level of S100A2 is positively correlated with LAMP3. (G) Representative immunofluorescence images illustrating the interaction between thyrocytes and cDC_C3 in two groups (A1 and B9). The small panels show the magnification of the selected region highlighted in red. Scale bars correspond to 50 µm in the large panel. (H) Schematic showing the crosstalk among thyrocytes, LAMP3 + DCs, CD8 + T cells, and Tregs involved in the recruitment of immune cells and the formation of an immunosuppressive microenvironment in the progressive PTC. cDC. conventional DC; DC, dendritic cell; CTLA-4, cytotoxic T-lymphocyte associated protein 4; ICAM, intercellular adhesion molecule 1; LAMP3, lysosomal associated membrane protein 3; PTC, papillary thyroid cancer; SPN, sialophorin; TME, tumor microenvironment; Treg, regulatory T cell.

Article Snippet: The following antibodies were used in the current study: primary antibodies against DC_LAMP/CD208 (1:400, CST, Cat#47778), CD8A (1:100 dilution; Abcam, Cat#ab217344), TIGIT (1:1,000, Cell Signaling Technology, Cat#99567T), NECTIN2 (1:400, Cell Signaling Technology, Cat#95333T), NECTIN3 (1:500, R&D, Cat#AF3064-SP), CCL17 (1:1,000, Abcam, Cat#ab195044), KRT19 (1:2,000, Abcam, Cat#ab76539), FOXP3 (1:2,000, Abcam, Cat#ab215206), or CCR4 (1:2,000, Novus Biologicals, Cat#NBP1-86584).

Techniques: Comparison, Expressing, Immunofluorescence, Membrane

Journal: Cancer Genomics & Proteomics

Article Title: Analysis of Protein–Protein Interactions Identifies NECTIN2 as a Target of N,N-Bis (5-Ethyl-2-hydroxybenzyl) Methylamine for Inhibition of Lung Cancer Metastasis

doi: 10.21873/cgp.20347

Figure Lengend Snippet: Note: The GO system reported NECTIN2 as poliovirus receptor-related 2 (PVRL2).

Article Snippet: The primary antibodies against NECTIN2 (#95333), β-catenin (#8480), vimentin (#5741), snail family transcriptional repressor 1 (SNAI1) (#3879), snail family transcriptional repressor 2 (SNAI2) (#9585), tight junction protein 1 (TJP1) (#8193), β-actin (#4970), and the secondary antibody anti-rabbit IgG (#7074) were acquired from Cell Signaling Technology (Danvers, MA, USA).

Techniques: